Mobilization of stem cell with granulocyte-colony stimulating factor promotes recovery after traumatic brain injury in rat
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Abstract:
Introduction: This study was designed to investigate the effects of granulocyte colony-stimulating factor (G-CSF) administration in rats for 6 weeks after traumatic brain injury (TBI). Methods: Adult male Wistar rats (n = 30) were injured with controlled cortical impact device and divided into four groups. The treatment groups (n = 10 each) were injected subcutaneously with recombinant human G-CSF. Vehicle group (n=10) received phosphate buffered saline (PBS) and only Brdu intraperitoneally. Bromodeoxyuridine (BrdU) was used for mitotic labeling. Experimental rats were injected intraperitoneally with BrdU. Rats were killed at 6th week after traumatic brain injury. Neurological functional evaluation of animals was performed before and after injury using neurological severity scores (NSS). Animals were sacrificed 42 days after TBI and brain sections were stained using Brdu immunohistochemistry. Results: Statistically significant improvement in functional outcome was observed in treatment groups when compared with control (p<0.01). This benefit was visible 7 days after TBI and persisted until 42 days (end of trial). Histological analysis showed that Brdu cell positive was more in the lesion boundary zone at treatment animal group than all injected animals. Discussion: We believe that G-CSF therapeutic protocol reported here represents an attractive strategy for the development of a clinically significant noninvasive traumatic brain injury therapy.
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mobilization of stem cell with granulocyte-colony stimulating factor promotes recovery after traumatic brain injury in rat
introduction: this study was designed to investigate the effects of granulocyte colony-stimulating factor (g-csf) administration in rats for 6 weeks after traumatic brain injury (tbi). methods: adult male wistar rats (n = 30) were injured with controlled cortical impact device and divided into four groups. the treatment groups (n = 10 each) were injected subcutaneously with recombinant human g-...
full textMobilization of Stem Cell With Granulocyte-Colony Stimulating Factor Promotes Recovery After Traumatic Brain Injury in Rat
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Traumatic brain injury (TBI) is described as a situation in which the brain is damaged by an external force. It is considered to be a chief problem in health care. Pharmacotherapy and stem cell therapy are the main treatments used in TBI. The purpose of these procedures is to control the inflammation in injured regions of brain. Currently, there is no effective care for TBI that could regenerat...
full textCombination of Stem Cell Mobilized by Granulocyte-Colony Stimulating Factor and Human Umbilical Cord Matrix Stem Cell: Therapy of Traumatic Brain Injury in Rats
Objective(s) Clinical studies of treating traumatic brain injury (TBI) with autologous adult stem cells led us to examine the impression of a combination therapy. This was performed by intravenous injection of human umbilical cord matrix stem cell (hUCMSC-Wharton,s jelly stem cell) with bone marrow cell mobilized by granulocytecolony stimulating factor (G-CSF) in rats injured with cortical com...
full textComparison of transplantation of bone marrow stromal cells (BMSC) and stem cell mobilization by granulocyte colony stimulating factor after traumatic brain injury in rat.
BACKGROUND Recent clinical studies of treating traumatic brain injury (TBI) with autologous adult stem cells led us to compare effect of intravenous injection of bone marrow mesenchymal stem cells (BMSC) and bone marrow hematopoietic stem cell mobilization, induced by granulocyte colony stimulating factor (G-CSF), in rats with a cortical compact device. METHODS Forty adult male Wistar rats we...
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Journal title
volume 1 issue 2
pages 37- 42
publication date 2010-01
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